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                       TLC and HPTLC

7 comparing lin /circ

comparing lin

Figure 7 : It looks like the maximum number of samples per plate
 can be separated by anti circular TLC. This is true. Per plate we could easily separate 48 samples on 100 x 100 mm plates but only when using computerized circular samplers. These tools existed only as test models. It was too early in the seventieth, at that time mass data analysis was not popular as at that time laboratory computers had not enough memory. Now - from on the years, when tera byte memory systems became payable and gigabyte ROMs are standard mass data handling in planar chromatography is not any more limited. By µ-PLC sampling techniques it is possible to test 400 samples per plate simultaneously. Not by scanners, they are since years outdated, but by digital cameras and photo software. In this case by linear multi integration.

The runs above are not yet done. The linear separation will stop when the mobile phase fronts from both sides touch each other. Immediately then the plate mast be taken and dried as under the mobile phase vapor bombardment the spots or lines diffuse much faster than by the original diffusivity. The circular run stops only by removing the mobile phase source. The anti circular runs stops also when there is no more free dry stationary phase available. In the center of the plate you will realize the concentrated “dirt” of the stationary phase. Click onto the right arrow icon. When any re run is done you will realize that the second start position MUST be accurately the same as at the first start. Accuracy and precisiopn in planar chromatography is necessary also for all mechanical tools.

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