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Inst.f.Chromatography

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                       TLC and HPTLC

23 partition coeff.

partition coeff

Figure 23 : The partition coefficient k in HPLC is helpful only
 in case of isocratic separation conditions, but the majority of HPLC separation are done programmed. Never the less: it may be helpful to see how the k(HPLC) data correlate with k(PLC) data. Well done and correctly measured the agreement is quite good. NOTE: linear Rf values only in TLC.

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